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  Česky / Czech version Čes. a Slov. Farm. 48, 1999, No. 3, p. 136–139
 
Examination of the Activity of Some Aminopeptidases in Immobilized Cells of the Poppy Plant 
STANO J. 1 , NEUBERT K. 2 , HEGEDÜS L. 3 , ANDRIAMAINTY F. 4 

 


Summary:

       Suspension cultures of the poppy plant (Papaver somniferum L.) were permeated with Tween 80 and immobilized with glutaraldehyde. The values of optimum pH of L-alanine aminopeptidase (L-Ala-AP), L-tyrosine aminopeptidase (L-Tyr-AP) and L-arginine aminopeptidase (L-Arg-AP) in native and immobilized cells are 8.0; 7.0 and 7.8, respectively. In the cells immobilized with glutaraldehyde, the activity of the enzymes under study is very low in comparison with native cells. In the immobilization of amino peptidases, alginate gel was successfully applied.

        Key words: amino peptidases – suspension cell cultures – immobilization with glutaraldehyde and alginate – Papaver somniferum
       

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