The present paper surveys the published HPLC methods estimating derivatives of acetic acid in biological materials and an HPLC method for estimation of indomethacine in the samples of whole blood. The elaborated chromatographic conditions and process of isolation were applied also to the analysis of tropesine and both drugs were determined simultaneously. The blood samples were adjusted by liquid-liquid extraction and indomethacine (and tropesine) was analysed on the reverse phase Separon SGX C-18 with the mobile phase methanol – water (pH 3.2) and detected at 254 nm. The method was used in a pharmacokinetic study of indomethacine on rabbits.

        Key words: HPLC – review of acetic acid derivatives – indomethacine – tropesine – blood sample – pharmacokinetics