The paper describes a method for porcine insulin labelling with technetium ( 99m Tc). When preparing this peptide, an insoluble redox polymer on the basis of cross-linked dextran (RP G25-IDA) was used for labelling. The prepared kits were labelled with 99m Tc with a high labelling efficiency (more than 95 %) and stability, as assessed using methods of chromatography. 99m Tc-insulin biodistribution in rats with experimentally induced diabetes – compared with a control group – did not demonstrate specific accumulation of the labelled substance in any organ. The method for kit preparation and its labelling are mild, HPLC and SEC did not reveal any products of insulin degradation and insulin content met the standards of Ph.B. 97. This is the first case of insulin labelling with techne- tium( 99 Tc) and, also, the first use of a redox polymer for this purpose.

        Key words: insulin – redox polymer – technetium ( 99m Tc) – radioassay – biodistribution