The paper presents the results obtained with the use of two chiral stationary phases (CSP) based on a macromolecular antibiotic agent – teikoplanin containing saccharide parts (CHIROBIOTIC T) and teikoplanin without saccharide parts (CHIROBIOTIC TAG). Racemic mixtures of 1-methyl-2- -piperidinoethyl esters of 2-, 3-, and 4-alkoxyphenylcarbamic acids were examined. The investigation included interactions between separated substances and CSP, and the effect of separation of the enantiomers under study on the value of the differentiation factor (Rij) under identical chromatographic conditions with the use of the method of high-performance liquid chromatography (HPLC). On the basis of the obtained results, it is possible to report that CSP-CHIROBIOTIC TAG is more advantageous for the substances of the type of 1-methyl-2-piperidinoethyl esters of 2-, 3-, and 4-alkoxyphenylcarbamic acids, because it does not contain a saccharide part, which decreases the possibility of non-polar interactions which exert a negative effect on the Rij value.

        Key words: teikoplanin – TAG – plenylcarbamic acids – HPLC – separation – separation of enantiomers – local anaesthetics