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CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ |
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| Česky / Czech version | Klin. Biochem. Metab. 10 (31), 2002, No. 4, p. 235–237 |
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Modification of HPLC Assay for
5-hydroxyindoleaceticAcid Estimationin Urine Resolved the Interference inTreatment
with 5-aminosalicylic Acid Dastych M.1, Dastych M. jr.2, Sojková V.1, Malášková L.1 1Oddělení klinické biochemie a hematologie FN Brno 2Interní gastroenterologická klinika FN Brno |
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Summary: The interference of assessment of 5-hydroxyindole-3-acetic acid in urine by the HPLC assay with electrochemical
detection can be caused by the aminosalicylate treatment. The separation of the interfering peak was
achieved by a change of the pH of the mobile buffer phase from the value 2.6 to 3.2.
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