Authors characterized skin microflora in 51 patients with acne after treatment either with vaccine or antibiotics. They also verified genetic homology of phenotypically identical isolates repetitively taken from the samé patient and determined selected virulence factors in Staphylococcus aureus species. Study confirmed participation of staphylococci and propionibacteriae in etiopathogenesis of acne and documented statistically not significant influence of both treatment modalities on the above mentioned pathogens. Sensitivity tests confirmed only low resistance of Propionibacteríum acnes to klindamycin (6.7%) and higher resistance of Staphylococcus epidermidis species (73.4% to erythromycin and 62.2% to klindamycin). Significant resistance changes were not demonstrated during study. Identical phenotype of coagulase negative staphylococci strains was found in 12 patients. Electrophore detekotic DNA profile analysis revealed genetic homology in 6 (50%) cases. Virulence factors detection demonstrated presence of gene for Panton-Valentine leukocidine synthesis in 3 Staphylococcus aureusstrains. No enterotoxin A and TSST-1 genes were found.

        Key words: acne – Propionibacterium acnes – Staphylococcus sp. – antibiotic resistance – factors of virulence