Verifying of S100B and
NSE reference interval – age, gender and sampling time dependence
Pikner R.1, Lavička P.2, Kormunda S.3, Topolčan O.4, Bosman R.5, Chytra I.5, Holubec L.4, Choc M.2
1Oddělení klinických laboratoří, Klatovská nemocnice, a. s. 2Neurochirurgické oddělení FN Plzeň 3Ústav sociálního lékařství, LF UK Plzeň 4Oddělení nukleární medicíny, úsek imunodiagnostiky FN Plzeň 5Anesteziologicko-resuscitační klinika FN Plzeň |
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Summary:
Objective: S100B protein and neuron specific enolase (NSE) are nowadays intensively investigated as markers of
central nervous system damage. Verification of reference values and result variability (biological) are basic conditions
of results interpretation.
Material and Methods: Reference intervals were verified on 50 volunteers, from them 10 had done repeatedly blood
sampling after 6 and 24 hours from baseline. The influence of age, sex and time of sampling on reference cut offs were
than analysed. Cut-offs were established as 95% percentile of marker level in analysed group.
Results and conclusion: We found that, NSE and S100B levels do not show any clinically important dependence on the
time of sampling, age and sex. NSE levels appear to have higher result variability and approximately 8% of samples
had to be excluded from analysis due to visible haemolysis. We established a higher reference cut off for S100B –
0.2 μg/l, than manufacturer had set up, but it did not have any crucial effect on clinical evaluation of results.
Key words:
NSE, S100B protein, reference interval, serum.
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