The Cell Immunophenotyping of the Skin Tissue of the Healthy Donors by the Flow
Cytometry
Petrisková J.1, Vrlík M.1, Péč M.2, Péč J.3
1Martinské centrum imunológie, Martin primár MUDr. Mojmír Vrlík, CSc. 2Ústav biológie, Jesseniova lekárska fakulta, Martin prednosta RNDr. Jozef Dobiaš, CSc. 3Dermatovenerologická klinika, Martinská fakultná nemocnica, Jesseniova lekárska fakulta, Martin, Slovenská republika prednosta Doc. MUDr. Juraj Péč, CSc. |
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Summary:
The aim of the recent study was to investigate the occurrence of the lymphocytes subpopulations
in a skin tissue of the healthy donors. We investigated the skin biopsies of 16 patients without any
skin diseases and without any immunomodulation treatment. There was found relatively law
presence of lymphocytes (1 %) in the skin samples. There were found T-lymphocytes almost exclusively.
In the skin samples percentage of B-cells (CD19+), naturals killers (CD16+CD56+), helper/inducer
T-lymphocytes (CD3+CD4+), suppressor/cytotoxic T-lymphocytes (CD3+CD8+), CD4/CD8 ratio,
activated T-lymphocytes (CD3+HLA-DR+, CD3+CD25+, CD3+CD69+), naive T-lymphocytes
CD4+CD45RA+) was determined. Compared with peripheral blood there was an increase of some of
the activation markers on the surface of the skin cells, what may be probably connected with
a continuous touch of the skin with the antigens of an external environment.
Key words:
immunophenotyping - skin tissue - healthy donors - flow cytometry
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