Objective: Presentation of the work of the Human Milk Bank in the Institute for the Care of Mother and Child in 1991 - 2000. Type of study: Retrospective analysis of data and results of ten years’ activity and description of the approved procedure of collection, processing and use of human milk provided by donors. Method: Mathematical analysis of assembled results. Milk processing: The authors describe the procedure of the organization of human milk donorship and the approved procedure of collection, processing, distribution and use of human milk. This procedure has been used without causing any problems for several years in the Human Milk Bank of the Institute for the Care of Mother and Child. Results: In the course of 10 years there were 327 women donors of humanmilk in our bank. During this period a total of 8747 litres of human milk from donors was processed and used. This corresponds to a mean annual amount of 874.7 litres. The mean age of the donors during this period was 27.22 ± 4.75 years. Part of the brief paper is also a review of the recommended and approved procedure of collection, processing and use of human milk from donors. Conclusion: The objective of the Human Milk Bank is to ensure adequate nutrition for those neonates and infants whose mothers for various reasons (on the part of the mother or infant) are unable to breastfeed their own child. The presented results give an account of ten years activity of the Human Milk Bank in the Institute for the Care of Mother and Child in Prague and of the achieved results - number of donors, amount of collected milk.

        Key words: human milk, breastfeeding, Human Milk Bank, collection and processing of human milk, organization of donorship