The application of 2,4-dinitrophenol and 2,6-dinitrophenol as reagents for a ion pair extraction spectrophotometry
was tested. The ion pair extraction spectrophotometry experiments were carried out using a simplified
procedure (test tube method). Four cationic tensides were used as model analytes. After the optimal conditions
of the assay were being determined, the influence of the concentration excess of the reagents on the absorbance
of the chloroform extract was investigated. Calibration curves were constructed; an estimation of limits of
quantitation was performed and apparent molar absorption coefficients were determined. The obtained results
show that both investigated dinitrophenols are suitable for the studied purpose.
dinitrophenol – extraction spectrophotometry – ion pair – tenside