Elicitation is based on signal (elicitor)-induced expression of genes, which subsequently results in
increased synthesis of secondary metabolities in plants and in vitro cultures. Elicitors do not usually
influence gene activity directly but by means of transmitters of the signal, which include primarily
calcium ions. The paper examined the effect of four concentrations of calcium ions on the production
of anthracene derivatives by means of the callus and suspension cultures of Rheum palmatum L.
(Polygonaceae), which was elicited by an abiotic elicitor – a 20 µM solution of lead dichloride. The
culture was cultivated on a Murashige-Skoog medium with an addition of 10 mg.l-1 of α-naphthylacetic
acid. It follows from the results that the maximal content of anthracene derivatives, determined
by the photometric determination according to PhBs 4, was demonstrated in the suspension
culture after 24-hr administration of the elicitor and a 10 mM calcium chloride solution. In
comparison with the control and elicited groups, the production was stimulated by 44 % and 17 %,
respectively. After an addition of calcium ions to the elicited callus culture, no positive influence on
the production was observed.
Rheum palmatum L. in vitro – anthracene derivatives – abiotic elicitation –