IL-6 and DRD2 Genes
are Associated with Hyperkinetic Disorder
Šerý O., Theiner P.1, Hladilová R., Štaif R., Balaštíková B.1, Drtílková I.1
Laboratoř neurobiologie a molekulární psychiatrie, Katedra srovnávací fyziologie živočichů a obecné zoologie PřF MU, Brno, vedoucí katedry prof. RNDr. V. Šimek, CSc. Psychiatrická klinika LF MU a FN, Brno, přednosta prof. MUDr. E. Češková, CSc. |
|
Summary:
TaqI A polymorphism of the dopamine receptor D2 gene (DRD2) was previously associated with
ADHD, alcoholism, drug dependence etc., while -174 polymorphism of interleukin-6 (IL-6) gene was
associated with alcoholism. In our association study, we studied the difference in genotype and
allelic frequencies of the above mentioned polymorphisms between boys with ADHD and boys in
control group. Buccal tissue was used for isolation of genomic DNA using commercial kit UltraClean
Tissue DNA (MoBio, USA). PCR with subsequent restriction analysis was used for determination of
genotypes. Genotype and allelic frequency of TaqI A polymorphism of DRD2 gene was statistically
different between ADHD group and control group (P < 0.04 and P < 0.002, respectively), as well as
genotype and allelic frequency of -174 polymorphism of IL-6 was statistically different between
studied groups of boys (P < 0.004 and P < 0.001, respectively). Our study confi rmed the association
found previously between TaqI A polymorphism and ADHD and provided new data about the fi rst
discovered association between -174 polymorphism of IL-6 gene and ADHD.
Key words:
ADHD, IL-6, DRD2, association study, polymorphism.
|
