The effect of potential precursors (cinnamic acid, phenylalanine, tyrosine) in three concentrations (0.01; 0.1; 1 mmoUl) on the growth of the culture and coumarin production was investigated in the suspension culture of Angelica archangelica L. The cultures esere cultivated under constant illumination (35001ux) and in the dark. The growth of the culture in the dark was not affected by the precursors, cinnamic acid in a concentration of 1 mmoUl exerted toxic action. The growth of the culture cultivated under constant illumination was stimulated by tyrosine in a concentration of 0.1 and 1 mmoUl, and phenylalanine in a concentration of 0.1 mmol/1; phenylalanine and cinnamic acid in a concentration of 1 mmoUl inhibited the growth of the culture. In the cultivation in the dark, coumarin production was increased by phenylalanine in a concentration 0.01 mmol/1 and by tyrosine in a concentration of 1 mmoUl. In the cultivation under constant illumination, coumarin production was decreased by the action of cinnamic acid, was not affected by tyrosine, and phenylalanine (0.01 and 0.1 mmoUl) increased it in comparison with the culture without precursors.
Angelica archangelica L. - suspension culture - coumarins - precursors - light