A flow injection-spectrophotometric determination at 540 nm of hydroxyamphetamine is reported. The procedure is based on a coloured reaction with 2-hydrazono-3-methylbenzothiazole (MBTH) and ceric ammonium nitrate (Ce IV ) as the oxidation reagent. Under optimised conditions, the determination of hydroxyamphetamine is achieved in a range of 0.01–0.2 mmol.l -1 with a relative standard deviation of 0.78 % for 0.05 mmol.l -1 (n=10) and a sample throughput of 75.h -1. The method was used for the determination of medicinal drug in the mass-produced dosage form of Czech origin: Pedrolon drops, Galena a.c.

        Key words: hydroxyamphetamine – Ce IV – flow injection analysis – spectrophotometric determination – drops