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  Česky / Czech version Čes. slov. Farm. 52, 2003, č. 4, s. 186–188
 
Effect of Auxins on the Production of Coumarins in the Suspension Culture of Angelica archangelica L. 
SIATKA T., KAŠPAROVÁ M. 

Katedra farmakognozie Farmaceutické fakulty Univerzity Karlovy, Hradec Králové
 


Summary:

       The paper examined the effect of selected auxins (2,4-dichlorophenoxyacetic acid, α-naphthaleneacetic acid, β-indoleacetic acid, β-indoleburytic acid; each in four concentrations – 0.2, 2, 10, and 20 mg/l) on the production of coumarins in the suspension culture of Angelica archangelica L. cultinated in the dark and under permanent lighting (3500 lux). The effect of the light regimen is, in comparison with auxins, less marked – the content of coumarins is mostly comparable both under permanent lighting and in the dark. The highest coumarin content was achieved with the use of α-naphthaleneacetic acid in a concentration of 0.2 mg/l with cultivation in the dark.

        Key words: Angelica archangelica L. – suspension culture – coumarin production – auxins – light conditions – flow injection analysis
       

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