The paper presents the results obtained with the use of two chiral stationary phases (CSP) based
on a macromolecular antibiotic agent – teikoplanin containing saccharide parts (CHIROBIOTIC T)
and teikoplanin without saccharide parts (CHIROBIOTIC TAG). Racemic mixtures of 1-methyl-2-
-piperidinoethyl esters of 2-, 3-, and 4-alkoxyphenylcarbamic acids were examined. The investigation
included interactions between separated substances and CSP, and the effect of separation of the
enantiomers under study on the value of the differentiation factor (Rij) under identical chromatographic
conditions with the use of the method of high-performance liquid chromatography (HPLC).
On the basis of the obtained results, it is possible to report that CSP-CHIROBIOTIC TAG is more
advantageous for the substances of the type of 1-methyl-2-piperidinoethyl esters of 2-, 3-, and
4-alkoxyphenylcarbamic acids, because it does not contain a saccharide part, which decreases the
possibility of non-polar interactions which exert a negative effect on the Rij value.
teikoplanin – TAG – plenylcarbamic acids – HPLC – separation – separation of
enantiomers – local anaesthetics