Bergenia crassifolia (L.) Fritsch in vitro
Sterile germinating plants were used to derive a tissue culture. The greatest stimulating effect on
the growth of the culture was exerted by NAA cultures in all concentrations tested, by IAA in
concentrations of 1.0 and 10.0 mg.l -1 , by IBA in a concentration of 0.1 mg.l -1 , and by the combination
of IBA + K. The difference between the values of growth on these media was statistically insignifi-
cant. TLC analysis of callus extracts demonstrated the presence of bergenin, arbutin, hydroquinone,
and methylarbutine. HPLC analysis confirmed the findings (arbutin 0.25 %, hydroquinone 0.05 %,
methylarbutin 0.28 %). The results of biotransformation tests show that the highest increase in
arbutin took place after addition of 4-hydroxybenzoic acid and withdrawal of the sample after 24
hours (3.82 %). No transformation of arbutin or 4-methoxyphenol to produce methylarbutin took
place in the culture under the given conditions. The highest increase in the summary content of
phenolic substances occurred with the use of the elicitor Pseudomonas aeruginosa – 88 % (conc.
0.0001 g/100 ml, 12-hour action).
tissue culture – Bergenia crassifolia – biotransformation – elicitation