Background. The disbalance of proteinase production and their inhibitors leads to destruction of tissues in many
pathological processes. Recently it was shown that the expression of proteinases is regulated also by interactions of
cells which is mediated by adhesive molecules. We wanted to find out whether this mechanism is involved also in
the destruction of joints in osteoarthritis.
Methods and Results. Cartilage, synovial and subchondral bone tissues, and synovial fluids were obtained from
23 joints after total endoprosthesis surgery. The solid tissues were extracted by TRIS buffer. The investigated protein
concentrations were assessed imunochemicaly. In all specimens gelatinase A, gelatinase B, stromelysin-1, TIMP-1,
soluble adhesive molecules sICAM-1 and sVCAM-1 and cytokines TNF-α and IL-8 were found. In cartilage and
synovial fluid the proteolytic potential of metalloproteinases was balanced with high concentrations of their inhibitor
TIMP-1 (259.4±105.2 pmol/g protein vs 2343.8±637.5 pmol/g protein in synovial fluid, p<0.00001, and 178.9±175.7
pmol/g dry weight vs 647.2±561.3 pmol/g dry weight in cartilage, p<0.001) but in synovial tissues and pathological
subchondral bones was not (257.4±617.2 pmol/g dry weight vs 171.3±170.8 pmol/g dry weight in synovium,
p=0.61716, and 17.4±15.4 pmol/g dry weight 33.6±33.3 pmol/g dry tissue in pathological subchondral bone,
Conclusion. From correlation analysis ensues that the bond of ICAM-1 and VCAM-1 on chondrocytes with
appropriate integrin ligands probably leads to up-regulation of gelatinase A and B and to down-regulation of TIMP-1.
Moreover it is apparent that TNF-α up-regulates both investigated adhesive molecules followed and stromelysin-1
osteoarthritis, adhesive molecules, cytokines, matrix metalloproteinases.