TaqI A Polymorphism of the DRD2 Gene and Alcoholism in the Czech Republic
Kašparovská J.*, Hříbková H.*, Hloušková R.*, Novotný M.***, Gláslová K.***, Šimek V.*, Mikeš V.**, Zvolský P.***, Šerý O.*
Přírodovědecká fakulta MU, Katedra srovnávací fyziologie živočichů a obecné zoologie, Laboratoř neurobiologie a molekulární psychiatrie, Brno, vedoucí katedry prof. RNDr. V. Šimek, CSc.* Přírodovědecká fakulta MU, Katedra biochemie, Brno, vedoucí katedry doc. RNDr. V.Mikeš, CSc.** Psychiatrická klinika 1. LF UK a VFN, Praha, přednosta prof. MUDr. J. Raboch, DrSc.*** |
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Summary:
Since 1990 many studies dealing with the association of the TaqI A polymorphism of the DRD2
gene with alcoholism and other psychic disorders were published. Controversial results of these
papers led us to investigate the relationship between TaqI A polymorphism of the DRD2 gene and
alcoholism in the Czech Republic. In this study 571 persons were included (280 alcoholics and 291
controls). The diagnostic criteria DSM-IV and ICD-10 for the diagnosis of alcoholism were used.
Genomic DNA from the blood using the commercial kit UltraClean Blood Spin kit (MoBio, USA)
was isolated. PCR and RFLP were used for genotyping. We found the same frequency of the A1
allele in alcoholic and controls (0,18). A statistically significant difference in the frequency of the
A1 allele was observed between males and females in the control group (p = 0.038). No association
of the Taq1 A polymorphism to alcohol dependence in the Czech Republic was found.
Key words:
TaqI A polymorphism, DRD2, alcoholism, alcohol dependence, association.
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