Background. Extracorporeal dialysis compensating kidney function represents a risk of elevated production of free radicals (FR). Paired filtration dialysis (PFD) is a hemodialysing method used to compensate kidney function. The aim of our work was to study effects of PFD with two types of hemodiafiltres on the activity of free radicals.
Method and Results. Group of nine regularly dialysed patients was treated with PFD 1) with demodiafilter SG3, composed of polysulphone high-flux hemodiafilter and hemophane low-flux dialysator, 2) with hemodiafilter SG30, composed of identical hemodiafilter as the above on and of a polysulphone low-flux dialysator. Parameters related to FR were examined before, at 30th minute and at the end of procedure. Plasma concentration of substances reacting with thiobarbiturate acid (TBARS) increased at 30th minute when PFD with SG3 was used (3.24±0,36 versus 3.48±0,31, p<0.01) and at the end of the procedure (3.24±0.36 versus 3.58±0.48, p<0.05). Glutathione values in erythrocytes (GSH) decreased at 30th minute of PFD with SG3 (1.85±0.27 versus 1.68±0.20, p<0.05). Plasma antioxidative capacity decreased at 30th minute and at the end of PFD with equal significance when either type of treatment was used (p<0.001). Glutathione peroxidase (GSHPx) decreased at 30th minute of PFD with SG30 (35.6±3.8 versus 32.2±3.1, p<0.05). Selenium (Se) in blood decreased at 30th minute (44.9±5.4 versus 40.4±5.9, p<0.05) and at the end of PFD with the same membrane (44.9±5.4 versus 39.4±5.2, p<0.05).
Conclusion. Elevation of TBARS and decrease of GSH show the presence of oxidative stress during the PFD treatment with hemodiafilter SG3. Changes probably result from the contact of blood with the hemophane membrane. The decrease of GSHPx during PFD with hemodiafilter SG30 is probably caused by the loss of Se. From the point of FR production, hemodiafilter SG30 can be considered as more advantageous, from the point of Se. From the point of FR production, hemodiafilter SG30 can be considered as more advantageous, from the point of Se loss and decreased activity of GSHPx, hemodiafilter SG3 has better effects.
hemodialysis, hemodiafiltration, paired filtration dialysis, free radicals, antioxidants, superoxide dismutase, glutathione peroxidase, glutathione, lipid peroxidation, selenium.