|CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ|
|Journals - Article|
|Česky / Czech version||Čes.slov.Farm.51,2002,No.4,p.182 –185|
Changes in Sanguinarine Content and Polyphenoloxidase Activity Due to a Fungal
Elicitor in Suspension Cultures of the Opium Plant Papaver somniferum L.
BALAŽOVÁ A.,BILKA F.,BLANÁRIKOVÁ V.,PŠENÁK M.
Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty Univerzity Komenského, Bratislava
The opium poppy (Papaver somniferum L.)is still a source for isolation of codeine and morphine.
Cell cultures from this plant lose their ability to produce morphinans.Their major alkaloid is
sanguinarine.The elicitation of the opium poppy cell cultures by fungal preparation lead to
a nine-fold increase in the content of sanguinarine.The specific activity of polyphenoloxidase (PPO)
was three-times higher in the elicited compared to the nonelicited cells.Two isoforms of PPO (Mr
63 kDa,41 kDa)were identified in opiumpoppy cell cultures by PAGE.The number of PPO isoforms
was not affected by elicitation.Phenyl-Sepharose CL-4B was used for affinity purification of PPO.
In a single purification step the specific activity of PPO was enriched 14-fold.
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