Background. Number of patients treated by general practitioners with various immunomodulatory drugs has
recently increased. Effects of such medication on the immune system were not usually monitored. The aim of our
work was to evaluate effect of selected immunomodulatory drugs on the phagocytic and metabolic activities of the
Methods and Results. 51 patients (18 males and 33 females) of the average age 36 years with repeating respiratory,
mycotic and herpetic infections were investigated. Immunomodulatory treatment included: Decaris (Lavamizolum),
Isoprinosine (Methisoprinolum), Imudon (Lysatum bacteriale mixtum), Biostim (Klebsiella pneumoniae), and
Immodin (Leukocyti dialysati lyophylysatum). Before and after treatment all patients underwent basic immunological
examination IgG, IgA, IgM, C3, C4 complement components, PEG, CD3, CD4, CD8 and CD19). Phagocytotic
activity was estimated by means of FAGO MSHP test with HEMA particles and by chemiluminiscence test.
Chemiluminiscence was measured using ML 3000 Microtiter Plate Luminometer (Dynex). 26 healthy individuals
of the corresponding age were the controls. Results were statistically evaluated by Student’s t-test. Significant
increase of the cellular metabolic activity was found in Decaris and Immodin treated patients (P<0.001).
Conclusions. Chemiluminiscence test, which evaluates the metabolic activity of phagocytes, can be used for the
accurate laboratory monitoring of the effects of some immunomodulatory drugs on the natural immunity of patients.
chemiluminiscence test, imunomodulatory drugs, phagocytes, cellular metabolic activity.