Our study focused on the effect of non-pharmacological intervention based on the modification of dietary habits and increasing physical activity on the level of total plasma cholesterol. Sample and Methods: Intervented sample was created by 279 highly motivated healthy adults from Brno (168 women and 111 men) of average age 43,5 ± 10,3 years and average level of total plasma cholesterol 6,1 ± 0,75 mmol/l and HDL-cholesterol 1,04 ± 0,14 mmol/l. After medical and life-style history assessment, followed by clinical and biochemical checking and evaluation the risk from the life-style and biochemical and clinical parameters, the participants were individually infor- med about recommendations. These recommendations regarded detailed changes of dietary ha- bits, quantified by the recommended number of servings of basic food groups and sub-groups daily and also increasing physical activity. Results: After 3 months the changes of biochemical parameters were evaluated. After our non- pharmacological intervention we founded significantly lower average level of total plasma choles- terol 5,36 ± 1,24 mmol/l (p < 0,001) and higher level of HDL-cholesterol 1,16 ± 0,14 mmol/l. The level of TG’s did not change significantly neither in whole sample, nor in the sub/sample of women and men. 26 % of our sample was resistant to the intervention (the difference in the total cholesterol level between 2 assessment was lower than 0,5 mmol/l). No significant difference was found between men and women regarding the reaction of plasma lipoproteins. Discussion and Conclusions: Our results justify the adequacy and appropriety of primary preven- tive advising focusing on decrease of the risk of premature death using non-pharmacological intervention in highly motivated people with good compliance and sufficient responsibility for their personal health.

        Key words: Non-pharmacological intervention - Dietary habits - Physical activity - Hyperlipopro-