We have studied tissue expression of the cytokine receptors using a high sensitivitity biotin-strep- tavidin system on cryostat sections. We used a panel of monoclonal antibodies from the 6th International Workshop on Human Leukocyte Differentiation Antigens, namely CD25 (IL-2Ra), CD95 (FAS antigen), CD116 (GM CSFR), CD117 (SCFR), CD120a (TNFR I), CD120b (TNFR II), CD121a (IL-1R I), CDw123 (IL-3R), CD124 (IL-4R), CD126 (IL-6R), CD127 (IL-7R), CDw128 (IL-8R), CD130 (gpl130), CD131 (IL-3R), CD132 (IL-2Rg), CD134 (OC-40), CD135 (FLT3/FLK2). Examined tissues (lymph nodes and spleens) were obtained from 12 patients with folicular non-Hodgkin´s lymphoma, periferal T non-Hodgkin´s lymphoma, B lymphoma, myeloma, Hodgkin´s disease, two cases of T cell rich B-lymphoma, autoimmune haemolytic anemia and two cases of rudimentary trombocytopenic purpura. Our results indicate that immunohistological technology using native tissues on cryostat sections, monoclonal antibodies and the visualisation with biotin-streptavidin is a particularly suitable supplementary staining procedure for detection of the cytokine recep- tors in tissues.

        Key words: cytokine receptors - cryostat tissue sections - biotin-streptavidin