Aim: Aim of the study was to prove possibilities of laparoscopic diagnostics of an acute bowel ischemia when using fluorescein dye and the ultra-violet (UV) light. Material and Methods: There were five animals (domestic pigs) included into the experiment in the year 2005. The endoluminal embolization of the peripheral branch of superior mesenteric artery (SMA) was made. Optical filters were placed to laparoscopic set to produce UV light. Fluorescein was administered intravenously and bowel inspection and applying the clips on the border of ischemia visualized by fluorescein was performed. Results: In all cases, the combination of laparoscopy, UV light and fluorescein dye distinguished ischemic part of bowel from the viable remnant. Conclusion: Combination of the UV light and fluorescein dye is able to reliable differentiate the viable segments of the bowel from the ischemic ones.

        Key words: bowel ischemia – laparoscopy – fluorescein – ultraviolet-light