Surface of the corneal stroma after microkeratome cut was assessed by classical and environmental SEM. Environmental SEM allows magnification of tissue without it´s previous drying. It is easy to prepare tissue for environmental SEM. Disadvantage of this leading technique is smaller scale of magnification and small angle of view. Classical SEM shows better the impact of LASIK on the cornea.

        Key words: environmental scanning electron microscopy, SEM, LASIK, cornea