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CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ |
| Journals - Article | |
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| Česky / Czech version | Transfuze a hematologie dnes, 8, 2002, No. 3, p. 91–95. O. |
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Preparation and Use of Tumor Antigen from Pathological Immunoglobulin
for Patients with Multiple Myeloma Musilová R.1, Táborská E.2, Bourková L.1, Büchler T.1,3, Hanák L.1, Kovářová L.1,3, Novotná H.4, Penka M.1, Hájek R.1,3 1Laboratoř experimentální hematologie a buněčné imunoterapie, Oddělení klinické hematologie, FN Brno, 2Lékařská fakulta MU, Katedra lékařské chemie a biochemie, 3Interní hematoonkologická klinika, FN Brno, 4Oddělení klinické biochemie, FN Brno |
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Summary: Idiotype vaccination can be used as immunotherapy for B-cell malignancies, including multiple
myeloma. Malignant plasma cells produce monoclonal immunoglobulin that can be detected in the
serum and/or urine and is tumour-specific. To isolate the myeloma Id protein we used affinity chromatography
on a protein G column. Id was eluted with 0.2M glycine-HCl. The protein was more than 90 %
pure as determined by SDS-polyacrylamide gel electrophoresis. Id protein was conjugated with KLH
in the presence of 0.1% glutaraldehyde. The conjugation product was administered in the preclinical
experiment to test vaccination as a part of the clinical protocol under preparation.
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