Possibilities of Age Assessment by Peptide Mapping of Non-collagenous Proteins of Human Dentin
Sajdok J.~, Kotrbová-Kozak A.1, Pilin A.2
lÚstav biochemie a mikrobiologie, Fakulta potravinářské a biochemické technologie,VŠCHT, Praha 2Ústav soudního lékařství a toxikologie 1. LF UK a VFN, Praha |
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Summary:
The submitted paper deals with possibilities of assessment of human age based on findings of stereospecific breakdown of proteins containing D-formy of aspartic acid.The stereospecificity of enzymatic breakdown assumes that after enzymatic hydrolysis peptide breakdown producta with different molecular weights - the so-called peptide map - will be formed, depending how many D-aspartyl residues the protein contains. The suthors proved in the submitted preliminary study in subjects of different age the formation of breakdown products of different size in non-collagenous proteins of human dentin which was hydrolyzed by protease VS from Staphylococcus aureus.
Key words:
assessment of human age, d-aspartic acid, non•collagenous dentin proteins, breakdown by protease from Staphylococus aureus, peptide map
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