Preimplantation Genetic Diagnosis (PGD) of
Chromosomal Aberrations by Fluorescent in situ
Hybridization (FISH) – Introduction to Problem,
Sampling Methods and Examination Technique
Veselá K., Tauwinklová G., Trávník P., Veselý J., Hlaváčová S., Zudová D.,Řezáčová O., Rybář R., Rubeš J.
1Sanatorium REPROMEDA, Brno, vedoucí lékař MUDr. J. Veselý, CSc. 2Výzkumný ústav veterinárního lékařství Brno, ředitel MVDr.M. Toman, CSc. |
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Summary:
Objective: Presentation of preimplantation genetic diagnosis and the set of laboratory processes like
aspiration, preparation and evaluation of polar bodies, sperm cells and blastomeres using FISH
method (fluorescent in situ hybridization) in ART.
Design: Review.
Setting: Sanatorium REPROMEDA, Brno, Veterinary Research Institute, Brno.
Methods: Overview of published data and own clinical experience with the cell aspiration methods,
evaluated sample preparation and the proper chromosomes visualisation using FISH method.
Conclusion: The review brings an overview of conditions and methods including sample obtaining,
FISH analysis preparation and implementation, processed during PGD.
Key words:
preimplantation genetic diagnosis, PGD, FISH, genetic factor, infertility
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