Summary:
Examination of chimerism has become an integral element of the monitoring of patients after allogenic
hematopoietic cell transplantation. Presented publication initially describes different methods of
chimerism evaluation. In the second part, there is detailed characterization of the capillary electrophoresis
with fluorescence detection (fragment analysis) of amplification products of high polymorfic
micro- and mini- satellite DNA regions, technique, which become the gold standard for quantitative
evaluation.
Key words:
chimerism, STR and VNTR polymorphism, fragment analysis
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