The purpose of this studywas to identify limbal stemcells in the culture of human donor cornea. After standard cultivation procedure we usedmonoclonal antibody against alpha-enolase for identification of stem cells. This antibody serves as a probe for the identification of biochemical marker of stem cells. Using immunofluorescent microscopy we identified in specimens
-enolase positive cells whichin vitro but not in situshowed alsosomemorphologic differencesfrom other cellular elements of the culture.

        Key words: limbal stem cells, alpha-enolase, immunofluorescent microscopy