Transplantation neural stem
cells and hematopoiesis reparation
Filip S.1, Mokrý J.2, Karbanová J.2, Vávrová J.3, Vokurková J.4
1Onkologická a radioterapeutická klinika FN, Hradec Králové 2Katedra histologie a embryologie LF UK, Hradec Králové 3Ústav imunologie a radiobiologie VLA JEP, Hradec Králové 4Odd. klinické imunologie FN, Hradec Králové |
|
Summary:
Stem cells for their unique property - plasticity promise a universal utilization,mainly, in the reparation
of most organs and tissues in the human body. In this communication, the authors presents their
experimental results in a model of neural stem cells (NCSc) where the main goal was to preserve the
NSCs hemopoietic identity. Mice given whole body sublethal irradiation with a dose of LD 8.5 Gy were
saved by transplantation of labelled (X-Gal+) NSCs. The demonstration of increased uptake of NSCs in
the spleen led us to study the relationship of hemopoietic microenvironment and hemopoietic identity.
The proof of hemopoeitic identity was performed by cultivation of CFU-GM from the bone marrow -
where cells exhibiting X-Gal positivity were identified by histochemistry. Our experiments show
different ways how NSCs transplantation may influence the damaged hematopoiesis.
Key words:
stem cells, transplantation, hematopoiesis, cell therapy
|
