Objective: The aim of this study was to compare levels of PGE2 in cervical ovulatory mucus in patients with various frequency of spontaneous miscarriages, and in healthy fertile women. Setting: Special Consultation for Immunology of Reproduction and Research Laboratories for Reproductive Immunology, Department of Gynaecology and Obstetrics, Medical Faculty of Charles University and Faculty Hospital, Pilsen. Methods: We used commercial, high sensitive method of ELISA (PGE2 HS, R&D Systems) for detection of PGE2 in cervical ovulatory mucus. Statistic analysis (T-test, ANOVA-test and Kruskal-Wallis test) also provided our study. Results: It was taken in total 120 measurements.The R&D Systems company does not mention physiological levels of PGE2 in cervical ovulatory mucus. Therefore we compared all results of our studied groups with average concentration (197.356 pg/ml PGE2) gained from control group.We found that in patients with one miscarriage PGE2 levels are approximately 6 times higher, with two miscarriages 13 times higher, and in patients with three and more miscarriages even 21 times higher than controls (p<0.0327, α 5%). Conclusion: In present literature does not exist any study concerning of PGE2 in cervical ovulatory mucus. We prove that some patients mainly with repeated „unexplained“ miscarriages have ovulatory levels of PGE2 significantly higher than controls.

        Key words: prostaglandin E2, cervical ovulatory mucus, miscarriage