Immunofluorescent Analysis of Anterior Lens Capsule Allotransplantation for
Chronic Corneal Ulcers
Kozák I.1, Trbolová A.2, Rosocha J.3, Jautová J.4, Juhás T.1, Ledecký V.
Očná klinika LF UPJŠ, Košice, prednosta doc. MUDr. T. Juhás, DrSc. Klinika chirurgie, ortopédie a roentgenológie, Univerzita Veterinárskeho lekárstva, Košice, prednosta doc. MUDr. V. Ledecký, CSc. Združená Tkanivová banka LF UPJŠ, Košice, prednosta MVDr. Ján Rosocha, CSc. Kožná klinika LF UPJŠ, Košice, prednostka doc. MUDr. J. Jautová, PhD. |
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Summary:
This study uses immunofluorescent analysis to find out in histologic corneal
sections a possible rejection reaction in anterior lens capsule allotransplantation
for chronic corneal ulcers. The experiment included control group of rabbit
corneas with repeat injury to epithelium and anterior stroma as well as surgically
treated group. Fluorescein-conjugated human antibodies to IgA, IgM, IgG, complement
and fibrinogen were used in the study. For detection we used method of
direct immunofluorescence. Positive was only examination on fibrinogen in sections
of treated group eyes 2 days after surgery. Last examination after 3 months
failed to detect positivity in any of the tested markers.We preliminarily conclude
that anterior lens capsule allotransplantation for chronic corneal ulcers does not
result in rejection reaction and that components of humoral immunity do not
participate in immune reaction.
Key words:
anterior lens capsule, allotransplantation, corneal ulcer, immunofluorescent
analysis
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