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CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ |
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Česky / Czech version | Vnitř. Lék., 50, 2004, No. 11, p. 842 - 845 |
Primary Biliary Cirrhosis - Specific Anti-mitochondrial Antibodies Wenchich L.1, Krechler T.2, Švestka T.2, Horák J.3, Šedivá A.4, Bartůňková J.4, Hansíková H.1, Martásek P.1, Zeman J.1 1Centrum integrované genomiky, Klinika dětského a dorostového lékařství 1. lékařské fakulty UK a VFN, Praha, přednosta doc. MUDr. J. Hoza, Csc. 2IV. interní klinika 1. lékařské fakulty UK a VFN, Praha, přednosta doc. MUDr. A. Žák, DrSc. 3I. interní klinika 3. lékařské fakulty UK a FN Královské Vinohrady, Praha, přednosta prof. MUDr. J. Horák, DrSc. 4Ústav imunologie 2. lékařské fakulty UK a FN Motol, Praha, přednosta prof. MUDr. J. Bartůňková, DrSc. |
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Summary: Primary biliary cirrhosis is a chronic liver disease, characterized by the destruction of the epithelial
cells of the sublobular, interlobular and septal bile ducts and with the development of cirrhosis.
The presence of anti-mitochondrial antibodies against the subunits of mitochondrial
2-oxoacids dehydrogenases is characteristic for patients with primary biliary cirrhosis. The aim
of this work was to study the effect of anti-mitochondrial antibodies upon activity of the isolated
mitochondrial pyruvate dehydrogenase complex after the incubation with serum from patients
with primary biliary cirrhosis. Group of patients and methods. The activity of the purified bovine
pyruvate dehydrogenase complex was studied spectrophotometrically in presence of the serum (1
: 1000) from five patients with primary biliary cirrhosis and from ten disease free controls. Results.
The activity of the pyruvate dehydrogenase was decreased after incubation with the serum
from patients with primary biliary cirrhosis. No similar inhibitory effect was found after incubation
with serum from controls. Discussion. The inhibitory effect of the anti-mitochondrial antibodies
upon activity of pyruvate dehydrogenase may broaden the spectrum of diagnostic methods in
patients with primary biliary cirrhosis. Further investigations are necessary to assess the possible
application of this method for monitoring of changes during the course of the disease and for
assignation of the disease prognosis.
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