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CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ |
| Journals - Article | |
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| ACTA CHIRURGIAE PLASTICAE 41, 4, 1999, pp. 120 - 122 | |
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A MODIFIED STAINING METHOD FOR CONNECTIVE
TISSUE IN SEMI-THIN HISTOLOGICAL SECTIONS
OF PERIPHERAL AND CRANIAL NERVES Menovsky T. 1 , van den Bergh Weerman M. 2 1 Department of Neurosurgery, University Hospital Nijmegen St. Radboud, Nijmegen, The Netherlands 2 Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands |
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Summary: A simple staining method for collagen in semi-thin sections of nerves is described. This technique consists of ap-
plying basic fuchsin (0.05%) solution for 2 min. to ± 1.25 mm plastic resin-embedded sections after routine toluid-
ine blue staining. This staining method clearly demonstrates the amount and orientation of collagenous
connective tissue in the nerve, both in transverse and longitudinal sections.
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