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  ACTA CHIRURGIAE PLASTICAE 41, 4, 1999, pp. 120 - 122
 
A MODIFIED STAINING METHOD FOR CONNECTIVE TISSUE IN SEMI-THIN HISTOLOGICAL SECTIONS OF PERIPHERAL AND CRANIAL NERVES 
Menovsky T. 1 , van den Bergh Weerman M. 2 

1 Department of Neurosurgery, University Hospital Nijmegen St. Radboud, Nijmegen, The Netherlands 2 Department of Pathology, Academic Medical Center, Amsterdam, The Netherlands
 


Summary:

       A simple staining method for collagen in semi-thin sections of nerves is described. This technique consists of ap- plying basic fuchsin (0.05%) solution for 2 min. to ± 1.25 mm plastic resin-embedded sections after routine toluid- ine blue staining. This staining method clearly demonstrates the amount and orientation of collagenous connective tissue in the nerve, both in transverse and longitudinal sections.

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