Objective: Detection of antizonal antibodies (aZPs) in cervical ovulatory mucus and in serum in patients with fertility failure. Design: Retrospective study. Setting: Department of Gynaecology and Obstetrics, Medical Faculty of Charles University, and Faculty Hospital, Plzeň. Methods: Detection of aZPs (passive haemagglutation test) in cervical ovulatory mucus (OM) and in serum from 105 patients with reproductive failure and in 25 fertile healthy women (control group). Results: Positive levels of aZP in OM were found in 79 (75.2%) patients, in 56 of them aZP were observed in OM and in serum together, only ovulatory aZPs in 23 (21.9%), and only serum aZPs in 18 (17.1%) were found. aZPs predominate in 47 patients (of 48 women) with more than two in vitro fertilization processes, in thirty of them aZPs in serum and in OM together were observed. On the onother hand, repeated spontaneous miscarriage and obesity have no connection with detection of aZPs in our patients. Conclusion: The increased levels of serum and OM aZPs are very often seen in patients after repeated usuccessful IVF. This very important result in reproductive imunology means to go on the micromanipulation technics combined with immunossuppression in the next planned IVF procedure.

        Key words: antizonal antibodies, fertility disorders