Comparison of Standard Prognostic Factors with the Deletion of 13q14
Detected by Interphase Fluorescence in Situ Hybridization on Separated and Unseparated Bone Marrow Cells in
Multiple Myeloma
1Smejkalová J., 4Vranová V., 3Oltová A., 4Kuglík P., 3Filková H., 1Heinigová J., 1Kovářová L., 2Adam Z., 2Krejčí M., 2Pour L., 1,2Büchler T., 5Svobodník A., 2Vostřejšová S., 2Kalábová V., 2Vorlíček J., 1Penka M., 1, 2Hájek R.
1Laboratoř experimentální hematologie a buněčné imunoterapie, Oddělení klinické hematologie FN, Brno 2Interní hematoonkologická klinik FN, Brno 3Oddělení lékařské genetiky FN, Brno 4Katedra genetiky a molekulární biologie, Přírodovědecká fakulta MU, Brno 5Centrum biostatistiky a analýz LF MU, Brno |
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Summary:
Background. Cytogenetic abnormalities of chromosome 13 are emerging as important prognostic factors in multiple
myeloma and have been associated with poor prognosis.
Methods and Results. The occurrence of 13q14 deletion and other standard laboratory parameters were determined
in 40 patients with multiple myeloma. We found that interphase fluorescence in situ hybridization using a locus
specific probe for RB1 gene on immunomagnetically selected myeloma cells was more sensitive than non selected
cells. The 13q14 deletion was found in 10 of 40 (25,0%) of bone marrow samples without cell selection and in 25 of
40 (62.5%) of samples with CD138+ enriched myeloma cells. Negative correlation was found between albumin and
the 13q14 deletion in separated (p=0,003) as well as in cells without selection (p=0,010). No significant correlation
was found in overall survival of separated and unseparated cells (p=0,830; p=0,260) and a similar result was obtained
for treatment response after transplantation of separated cells ( p=0,520) or non-separated cells (0,190).
Conclusions. Our results confirm that immunomagnetic selection of CD138+ cells increases the probability of
detection of the 13q14 deletion in bone marrow samples. The correlation was found between albumin and the 13q14
deletion in both of type of cells.
Key words:
multiple myeloma, magnetic-activated cell separation, interphase fluorescence in situ hybridization,
13q14 deletion, prognostic factors.
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