Summary:
In 179 samples of faeces from patients hospitalized with diarrhoeal diseases rotavirus infections
were examined concurrently by three different sets – the latex agglutination test Diarlex Rota-Adeno
(Orion Diagnostica) and two immunoenzyme tests (EIA) – EIA Rotavirus (Test-Line, CR) and
IDEIATM Rotavirus (Dako Ltd.) By the latex agglutination reaction the rotavirus infection was
proved 31 times (17.3%), by the Test-Line test 87 times (48.6%), by the Dako test 96 times (53.6%).
During the interval of 0–3 days after the onset of the disease the positivity assessed by different tests
was 22.8%, 48.6% and 54.3%, on the 4th–7th day 12.8%, 51.1% and 57.4%, on the 8th and subsequent
days 3.7%, 44.4% and 44.4%. Comparison of sets Orion Diagnostica and Dako revealed the sensitivity
of the latex agglutination test only as 30.2% and specificity 97.6%. After the 9th day from the onset
of the disease the infection was no longer confirmed by the latex agglutination test, while both EIA
tests proved the rotavirus antigen up to the 16th day from the onset of the disease.
Key words:
rotaviruses – latex agglutination – EIA – diagnosis.
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