Criteria for Evaluation of the Immunoblot in the Diagnosis of Lyme
Borreliosis Using Borrelia afzelii, Borrelia garinii and Borrelia burgdorferi sensu
stricto
Honegr K. 1 , Havlasová J. 2 , Gebouský P. 1 , Dostál V. 1 , Pellantová V. 1 , Škrabková Z., 3, Hulínská D. 4
1 Infekční klinika FN, Hradec Králové, 2 Ústav klinické imunologie a alergologie FN, Hradec Králové, 3 Ústav lékařské biofyziky, Oddělení výpočetní techniky, LF UK, Hradec Králové, 4 Státní zdravotní ústav, Národní referenční laboratoř pro lymskou borreliózu, Praha |
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Summary:
The immunoblot was prepared from genotypes Borrelia afzelii (KC 90), Borrelia garinii (M 192) and
Borrelia burgdorferi sensu stricto (B 31). Sera of 63 patients with different forms of Lyme borreliosis
were examined and 40 healthy donors in the endemic area of the disease. In class IgM in the group
of patients significantly more frequently antibodies against OspC, p39, p41 B. afzelii, p39, p41, p66,
p83 B. garinii and OspC1, OspA, B. burgdorferi sensu stricto were found. In class IgG there were
antibodies against p39, p41, p93 B. afzelii, p14, p41, p93 B. garinii and OspA, OspC p93 B. burgdorferi
sensu lato. Based on the assembled results by means of discrimination analysis and logistic
regression the most suitable combinations of antigens for evaluation of immunoblots in different
genotypes were determined. Furthermore evaluation was suggested using a combination of antigens of several genotypes which led to an increased sensitivity and specificity of the immunoblot.
Tables were prepared for easier evaluation of newly examined sera samples.
Key words:
immunoblot – Lyme borreliosis – Borrelia burgdorferi sensu lato.
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