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CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ |
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Česky / Czech version | Čes. Stomat., roč. 106, 2005, č. 6, s. 17–21. |
Application of Restriction Analysis of the Gene for 16S rRNA for Verification of Species
Identity of Important Oral Microorganisms
II. Periodontal Microorganisms Novotná G.1, Janata J.1, Dušková J.2, Janatová T.2 1Mikrobiologický ústav AV ČR, Praha, ředitelka prof. RNDr. B. Říhová, DrSc. 2Výzkumný ústav stomatologický 1. LF UK a VFN, Praha, přednostka prof. MUDr. J. Dušková, DrSc. |
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Summary: The authors recommend a simple algorithm for the restriction-endonuclease analysis of genetic
material of microorganisms present on mucous membrane of oral cavity. The method provides a significantly more
precise species identification of bacteria associated with diseases of periodontium. The method is based on analysis
of the part of the gene for 16S rRNA and its use is therefore universal and may be combined with identification
of other clinically important organisms of oral cavity, e.g. viridans streptococci. The requirements of the method
for equipment and cost are not high, as it uses a limited number of restriction enzymes. Although the method
is comparable with classical biochemical methods as far as the cost is concerned, it represents an improvement
due to unequivocal classification of the microorganisms, which is a general feature of molecular-genetic techniques.
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