From 1997 to 2000 in the Slovak Republic S Strains of Salmonella enterica Serovar Typhimurium DT104 were isolated from sporadic cases of human sahnonellosis. Four Strains were multiresistant and three Strains were sensitive to antibiotics. We investigated the influence of the cultivation medium (complete Miieller-Hinton, synthetic-Staples) on growth, surface hydrophobicity and motility of the mentioned isolates. The hydrophobicity was evaluated by methods of adherence to the hydrocarbon xylene (BATH) in a salt-aggregation test with ammonium sulphate (SAT) and adherence to a plast. The growth of tested Strains in 24 hours was greater after cultivation in the complete medium, but the salmonellae grew considerably also in the synthetic medium where the only Bource of C and N was asparagine. Of the investigated characteristics the cultivation medium influenced most the adherence of isolates to xylene as after cultivation in a synthetic medium all isolates with the exception of two were hydrophobic. The motility of Strains was also greater after growth in this medium. Conversely cultivation in complete medium suppressed these properties of isolates. The cultivation medium did not influence the adherence of isolates to plasts and only a slight influence was observed on the salt aggregating properties of the investigated Strains.

        Key words: Salmonella enterica Serovar Typhimurium DR 104 - surface hydrophobicity - motility