Objective: The aim of this study was to find out the behavior of sperm cryopreserved in microag- glutination test (TAT). Methods: TAT using native sperm pertains to the screening methods in spermagglutinating anti- bodies. Spermatozoa cryopreserved in egg-yolk medium, just in seminal plasma or in the commer- cial made medium - Medi - Cult (Denmark) were used in this study. Setting: Department of Gynaecology and Obstetrics, Institut of Clinical Immunology and Allergo- logy Medical Faculty of Charles University, Pilsen. Results: 367 tests were performed together. It includes 108 examinations with native spermatozoa, 59 with spermcryopreserved in egg-yolk medium, 109 with spermcryopreserved just in seminal plasma and 91 in Medi-Cult medium. The correspondance of results with native and cryopreser- ved sperm was found in 24% for egg-yolk medium, in 69% for seminal plasma and in 77% for commercial medium Medi-Cult. Conclusion: The results show that spermcryopreservation in Medi-Cult medium or just in the seminal plasma are able to be used in TAT for detection of spermagglutinating antibodies.

        Key words: microagglutinating test, sperm, cryopreservation, infertility