Non-invasive
fetal RHc genotyping from maternal peripheral blood
Hromadníková I.1, Doucha J.3, Benešová B.2 , Veselá K.1, Rožňáková E.2, Hakenová A.4
1Pediatrická klinika, 2Krevní banka, 3Gynekologicko-porodnická klinika, 2. LF UK a Fakultní nemocnice Motol, Praha, 4Transfuzní oddělení, Fakultní Thomayerova nemocnice, Praha |
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Summary:
In this prospective study, we described our first experience with non-invasive foetal RHc genotyping by analysis of
DNA extracted from maternal plasma samples by using real-time PCR, specific primers and TaqMan probe targeted
toward c allele of RHCE gene. We analysed 13 non-alloimmunised and 1 anti-c alloimmunised pregnant woman
within 12th and 33rd week of pregnancy and correlated the results with serological analysis of cord blood. Non-invasive
prenatal foetal RHc genotyping analysis of maternal plasma samples was in complete concordance with the analysis
of cord blood in all pregnancies. Non-invasive foetal RHc genotyping enables the identification of foetuses at
risk of haemolytic disease of the newborn. An identification of RHc negative foetuses in anti-c alloimmunized pregnancies
may exclude the demand of invasive prenatal procedures.
Key words:
foetal DNA, haemolytic disease of the newborn, quantitative real-time PCR, maternal plasma,
c allele, RHCE gene
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