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  Česky / Czech version Trans. Hemat. dnes, 11, 2005, No. 4, p. 14–16.
 
Non-invasive fetal RHc genotyping from maternal peripheral blood 
Hromadníková I.1, Doucha J.3, Benešová B.2 , Veselá K.1, Rožňáková E.2, Hakenová A.4 

1Pediatrická klinika, 2Krevní banka, 3Gynekologicko-porodnická klinika, 2. LF UK a Fakultní nemocnice Motol, Praha, 4Transfuzní oddělení, Fakultní Thomayerova nemocnice, Praha
 


Summary:

       In this prospective study, we described our first experience with non-invasive foetal RHc genotyping by analysis of DNA extracted from maternal plasma samples by using real-time PCR, specific primers and TaqMan probe targeted toward c allele of RHCE gene. We analysed 13 non-alloimmunised and 1 anti-c alloimmunised pregnant woman within 12th and 33rd week of pregnancy and correlated the results with serological analysis of cord blood. Non-invasive prenatal foetal RHc genotyping analysis of maternal plasma samples was in complete concordance with the analysis of cord blood in all pregnancies. Non-invasive foetal RHc genotyping enables the identification of foetuses at risk of haemolytic disease of the newborn. An identification of RHc negative foetuses in anti-c alloimmunized pregnancies may exclude the demand of invasive prenatal procedures.

        Key words: foetal DNA, haemolytic disease of the newborn, quantitative real-time PCR, maternal plasma, c allele, RHCE gene
       

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