In the period of 1994 – 1997, 4 552 samples of sectional, clinical material and environment were examined for the presence of Plesiomonas shigelloides. In 55 cases (1,21 %) Plesiomonas shigelloides was isolated. Within 55 isolated strains, 26 serovars have been successfully identified. Among other secured cases was Plesiomonas shigelloides isolated in pig (Sus scrofa domestica), mouse Mus musculus), binturong (Arctictis binturong) and raccoon (Pocyon lotor). In mandarin duck (Aix galericulata) the strain of Plesiomonas shigelloides with a new 0 antigen – 097 was isolated. In regard of the particular biological classes, the isolation of Plesiomonas shigelloides in fish (2.63 %) predominate, followed by reptiles (1.66 %), mammals (0.94 %) and birds (0.79 %). Plesiomonas shigelloides in amphibians was not demonstrated. This work surveys the incidence of Plesiomonas shigelloides and its serovars in animals in the region Moravia in the Czech Republic.

        Key words: Plesiomonas shigelloides, detection in wildlife animals