Immunohistochemical Characterization of Collagen IV in Control Corneas and in Corneas Obtained from Patients Suffering
from Posterior Polymorphous Corneal Dystrophy
Merjavá S.1, Lišková P.1, Jirsová K.1,2
1Laboratoř biologie a patologie oka, Institut dědičných metabolických chorob, VFN a 1. LF UK, Praha, vedoucí pracoviště prof. MUDr. Milan Elleder, DrSc. 2Oční tkáňová banka, VFN a UK, Praha, vedoucí pracoviště MUDr. Marcela Černická, MBA |
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Summary:
The expression of all six chains of collagen IV was studied using the indirect fluorescent immunohistochemistry in seven control
corneas and seven corneas obtained from patients suffering from the posterior polymorphous corneal dystrophy. Heterogeneous
staining, especially in the epithelial basement membrane and Descemet’s membrane, was observed in the control corneas.
An increase of the staining intensity for the α1 and α2 chains was observed, especially in the Descemet’s membrane and
the corneal stroma in samples obtained from the patients compared to the control tissues.
Key words:
collagen IV, cornea, indirect fluorescent immunohistochemistry
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