Presentation of b-Galactosidase Secretion by Plant Organs
STANO J., MIČIETA K. 1 , ULBRICH-HOFMANN R. 2 , HAVRÁNEK E. 3 , GRANČAI D. 4 ,
4 Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava 1 Katedra botaniky Prírodovedeckej fakulty Univerzity Komenského, Bratislava 2 Biochemický ústav Univerzity Martina Luthera, Halle/Salle 3 Katedra farmaceutickej analýzy a nukleárnej farmácie Farmaceutickej fakulty Univerzity 4 Komenského, Bratislava 4 Katedra farmakognózie a botaniky Farmaceutickej fakulty Univerzity Komenského, Bratislava 5 Klinika pracovného lekárstva a toxikológie, Bratislava 6 Katedra farmaceutickej chémie Farmaceutickej fakulty Univerzity Komenského, Bratislava 7 Katedra chemickej teórie liečiv Farmaceutickej fakulty Univerzity Komenského, Bratislava |
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Summary:
A simple, sensitive and reproducible method of detection of extracellular b-galactosidase was worked
out. b-Galactosidase secreted by the callus culture, or the roots of sprouting plants, hydrolyzes the
substrate (6-bromo-2-naphthyl-b-D-galactopyranoside) to produce b-D-galactose and 6-bromo-2-na -
phthol, which after simultaneous azocoupling with hexazotized p-rosaniline, or basic fuchsine,
produce a reddish brown compound, nearly insoluble in water (a diazonium salt). The colouring
under the callus culture and around it, as well as the colouring of the roots of the sprouting plants,
is a manifestation of the activity of extracellular b-galactosidase by the objects under study. The
intensity of the colouring is a measure of their enzymatic activity. The share of intracellularly
localized enzyme represents the majority and the share of extracellular b-galactosidase the minority.
Key words:
secretion of enzyme – carrot – extracellular b-galactosidase – diazonium salt
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