Organ Specific Antibodies in Children with Idiopathic Inflammatory
Bowel Disease and Their Employment in Clinical Practice
Sýkora J., Varvařovská J., Stožický F.,Haschová M.,Hanzlíková J.
Dětská klinika LF UK a FN, Plzeň Ústav imunologie a alergologie LF UK a FN, Plzeň |
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Abstract:
Background. Ethiopathogenesis of the idiopathic inflammatory bowel disease has not been yet fully explained.
Several abnormalities of the humoral immunity supporting the concept of autoimmune character of the disease have
been identified. The aim of our study was to characterise occurrence of the organ specific antibodies against the
intestinal goblet cells and against acinar cells of the exocrine pancreatic tissue and to evaluate their significance for
the diagnostics of the idiopathic inflammatory bowel disease.
Methods and Results. 69 children were included in the study. The group consisted of 20 patients with idiopathic
proctocolitis (11 boys and 9 girls, 6 to 18 years old, average age 15.5) and 14 patients with Crohns disease (9 boys
and 5 girls, 5 to 18 years old, average age 14.7). Control group included 35 children (20 boys and 15 girls, average
age 14.7). In patients of the idiopathic proctocolitis group, antibodies against the intestinal goblet cells were assayed
by indirect immunofluorescence method in 55%. In patients with Crohns disease, antibodies against acinar cells of
the exocrine pancreatic tissue were found in 64.2 %. Differences in manifestation of antibodies against acinar cells
of the exocrine pancreatic tissue in Crohns disease were statistically significant (p=0.001). Statistically significant
(p=0.01) was also the difference of levels of antibodies against the intestinal goblet cells in patients with idiopathic
proctocolitis when compared to patients with Crohns disease. Statistically significant difference (p=0.01) was found
in levels of antibodies against acinar cells of the exocrine pancreatic tissue in Crohns disease and antibodies against
the intestinal goblet cells in patients with idiopathic proctocolitis.
Conclusion. Testing on presence of specific antibodies against acinar cells of the exocrine pancreatic tissue and
against intestinal goblet cells is a valuable tool for the diagnostics of the idiopathic inflammatory bowel disease in
childhood and adolescence.
Key words:
idiopathic inflammatory bowel disease, idiopathic proctocolitis, Crohns disease, immunofluorescence,
specific antibodies, acinar cells of the exocrine pancreatic tissue, intestinal goblet cells.
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