CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ | |
Journals - Article | |
Česky / Czech version | Čes. a Slov. Farm. 47, 1998, No. 6, p. 271–272 |
A New Method of Detection of Plant Extracellular Proteolytic Enzymes J. STANO 1 , I. ŠAFAŘÍK 2 , P. KOVÁCS 3 , K. NEUBERT 4 , M. ŠAFAŘÍKOVÁ 2 1 Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava 2 Laboratoř biochemie a biotechnologie Ústavu ekologie krajiny České akademie věd, České Budějovice 3 Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty Univerzity Komenského, Bratislava 4 Biochemický ústav Univerzity Martina Luthera, Halle-Wittenberg |
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Summary: By means of an insoluble chromogenic substrate (black gelasphere), a simple, sensitive and repro- ducible method of detection of extracellular proteolytic enzymes was worked out. Extracellular proteolytic enzymes (produced by a callus or suspension culture) hydrolyse black gelasphere. Colouring around the callus culture and under it is a sign of the activity of extracellular proteolytic enzymes produced by the callus culture. The intensity of colouring of the cultivating medium is a measure of the activity of extracellular proteolytic enzymes of the suspension culture.
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