CzMA JEP Home page CZECH MEDICAL ASSOCIATION J. Ev. PURKYNĚ
Journals - Article
CzMA JEP Home page News About Assocation Publishing Division Medical Journals Searching Supplements Catalogue
 
  Česky / Czech version Čes. a Slov. Farm. 47, 1998, No. 6, p. 271–272
 
A New Method of Detection of Plant Extracellular Proteolytic Enzymes 
J. STANO 1 , I. ŠAFAŘÍK 2 , P. KOVÁCS 3 , K. NEUBERT 4 , M. ŠAFAŘÍKOVÁ 2 

1 Záhrada liečivých rastlín Farmaceutickej fakulty Univerzity Komenského, Bratislava
2 Laboratoř biochemie a biotechnologie Ústavu ekologie krajiny České akademie věd, České Budějovice
3 Katedra bunkovej a molekulárnej biológie liečiv Farmaceutickej fakulty Univerzity Komenského, Bratislava
4 Biochemický ústav Univerzity Martina Luthera, Halle-Wittenberg
 


Summary:

       By means of an insoluble chromogenic substrate (black gelasphere), a simple, sensitive and repro- ducible method of detection of extracellular proteolytic enzymes was worked out. Extracellular proteolytic enzymes (produced by a callus or suspension culture) hydrolyse black gelasphere. Colouring around the callus culture and under it is a sign of the activity of extracellular proteolytic enzymes produced by the callus culture. The intensity of colouring of the cultivating medium is a measure of the activity of extracellular proteolytic enzymes of the suspension culture.

        Key words: extracellular proteolytic enzymes – insoluble chromogenic substrate – black gelasphere – Chelidonium majus L.
       

Order this issue

  BACK TO CONTENTS  
 
 
| HOME PAGE | CODE PAGE | CZECH VERSION |
©  1998 - 2008 CZECH MEDICAL ASSOCIATION J. E. PURKYNĚ
Created by: NT Servis, s.r.o., hosted by P.E.S. consulting, s.r.o.
WEBMASTER